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Chemical Strategies to Protein Phosphorylation and Their Clinical Applications

Speaker: W. Andy Tao, PhD, Professor of Biochemistry, Professor of Chemical Biology and Analytical Chemistry, Department of Medicinal Chemistry and Molecular Pharmacology, College of Pharmacy, Purdue University Location: 402 N Blackford St. Indianapolis, IN 46202 LD 010

Protein kinases and their substrates comprise extensive signaling networks that regulate many diverse cellular functions. However, methods and techniques to systematically identify kinases directly responsible for specific phosphorylation events have remained elusive. Here we describe integrated proteomic strategies to dissect kinase networks in high throughput and demonstrate their applications in multiple systems. Our group has introduced a set of chemical tools and proteomics strategies to analyze protein phosphorylation, in particular, to identify direct kinase substrates and upstream kinases. Recently, for the first time, we identified thousands of phosphoproteins isolated from small volumes of plasma samples and quantitatively measured phosphoproteins that are significantly higher in patients diagnosed with breast cancer as compared to healthy controls. Our studies demonstrate that the development of phosphoproteins as disease biomarkers is highly feasible and may transform disease early detection and monitoring.  

Host: Webb