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Developing Electrochemical Sensors for Small Molecule Dynamic Monitoring

Speaker: Ryan White, PhD, Associate Professor, Ohio Eminent Scholar, Department of Chemistry, Department of Electrical Engineering and Computing Systems, University of Cincinnati Location: 402 N Blackford St. Indianapolis, IN 46202 LD 010

Monitoring small molecule dynamics in, for example, the brain represents a hefty analytical challenge that spans a broad dynamic range of temporal scales, size scales, and chemical space. Electrochemical, aptamer-based (E-AB) sensors provide a means to monitor, specifically and selectively, a broad range of small molecule targets. E-AB sensors are a class of sensors the rely on analyte-binding induced conformation changes of affinity reagents known as nucleic acid aptamers. In this talk, I will discuss our latest advances in aptamer-based sensors to push the temporal and spatial resolution to begin to address challenges in monitoring the dynamics of small molecules in the brain related to glial function. More specifically, we have developed analytical tools by pushing the limits of E-AB sensors to achieve ms-resolved detection at a size scale of a single cell. We have applied these sensors coupled with 3D tissue matrices to monitor the release of adenosine triphosphate from primary astrocytes in response to several stimuli. Furthermore, we have begun to push this detection down to the single cell level. Finally, I will discuss other how understanding the electrochemistry and biophysics of nucleic-acid tethered redox molecules help aid in our rational design of new sensing strategies.

Host: Deiss